An alginate lyase from Pseudomonas sp. KS408 was cloned and characterized. Recombinant E. coli harvested with alginate gene was expressed by IPTG induction and purified by using Ni-Sepharose affinity chromatography. Alginate oligomers obtained by degrading alginate with KS408 alginate lyase were analized by using FPLC and H-NMR. Peak profile from NMR showed that KS408 alginate lyase was polyM-specific lyase (β1-4 lyase, EC4.2.2.3). KS408 alginate lyase could cleavage between D-b-mannuronate and D-b-mannuronate linkage (MM) not D-b-mannuronate and L-α-guluronate linkage (MG). The molecular weight of oligoalginate products also analyzed with Electron-spray-ionization/Mass spectroscopy (ESI-MS). When polyM block was treated with KS408, four part of dimer and 1 part of trimer could be obtained. Acknowledgement : This work was supported by New &Renewable Energy R&D program(20093020090020) under the Korea Ministry of Knowledge Economy(MKE) and Korea Industrial Technology Foundation (KOTEF) through the Human Resource Training Project for Strategic Technology.
키워드
Alginate lyasepolyM specific lyaseD-mannuronateL-guluronate
저자
Yoo Jung PARK [ Department of Food Science and Biotechnology, Kyungsung University, Busan 608-736, Korea. ]
Eun Yeol LEE [ Department of Chemical Engineering, Kyung Hee University, 446-701, Korea. ]
Hee Sook KIM [ Department of Food Science and Biotechnology, Kyungsung University, Busan 608-736, Korea. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
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3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
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간행물
간행물명
한국생물공학회 학술대회
간기
반년간
수록기간
1985~2013
십진분류
KDC 476DDC 576
이 권호 내 다른 논문 / 한국생물공학회 학술대회 2011년도 한국생물공학회 춘계학술발표대회