A marine bacterium possessing a novel activity of alginate degradation was isolated from a brown seaweed soup liquefied by salted and fermented anchovy. The isolated strain was designated as Brevundimonas sp. MJ-3 based on the analysis of biochemical characteristics and cellular fatty acid composition. The alginate lyase gene was cloned from genomic DNA library and expressed in Escherichia coli. The optimal pH and temperature for the recombinant alginate lyase were 7.0 and 40oC, respectively. When the multiple sequence and conserved domain were analyzed, interestingly, the cloned gene product was predicted to consist of alginate lyase L-like protein domain and Heparinase-like protein domain. The final degradation product of alginate was analyzed by ESI-MS and proved to be unsaturated monosaccharides. Based on the results, the cloned gene possessed an exotype alginate lyase activity that can degrade the alginate into monosaccharides. Acknowledgement : This work was supported by New &Renewable Energy R&D program (20093020090020) under the Korea Ministry of Knowledge Economy (MKE) and Korea Industrial Technology Foundation (KOTEF) through the Human Resource Training Project for Strategic Technology.
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
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간행물
간행물명
한국생물공학회 학술대회
간기
반년간
수록기간
1985~2013
십진분류
KDC 476DDC 576
이 권호 내 다른 논문 / 한국생물공학회 학술대회 2011년도 한국생물공학회 춘계학술발표대회