Earticle

초록

영어

Pantoea ananatis carotenoid (PAC) is β-carotene biosynthesis gene. β -carotene is a carotenoid substance naturally found in plants that serves as an accessory to photosynthesis. It is primarily responsible for the pigment in orange colored fruits and vegetables, but also contributes to the pigment in red, yellow, and green colored fruits and vegetables. To express the PAC gene (2.95 kb) in Pleurotus eryngii, pPEV-PAC, which contains the CaMV 35S promoter, PAC gene, and nos terminator, was constructed and introduced into P. eryngii via Agrobacterium tumefaciens-mediated transformation. The correct integration of PAC gene in the genome of transgenic mycelium was confirmed by PCR amplification and sequencing. We also confirmed the transcriptional level of PAC gene for 1st and 2nd generation by RT-PCR. The mycelium harboring pPEV-PAC was grown at 25℃ for 7 days in mushroom complete medium and transferred on sawdust medium (sawdust and rice bran, 2:1) to induce fruiting body of P. eryngii under LED light (blue). Color variation of P. eryngii was observed on the gill of cultivated fruiting bodies. β-carotene levels in the fruit bodies were increased to approximately 2-fold than the control. Finally, we developed a new strain of P. eryngii with phenotypic expression of β-carotene. Accordingly, this system can be used for breeding new functional mushroom and the defensive, intellectual property for genetically modified organism (GMO) will be secured.

키워드

저자

• Dong Ju SHIN [ Dept. of Bioindustry, Daegu University, Kyungbuk, 712-714 ]
• Sun Hwa HA [ Dept. of Bioindustry, Daegu University, Kyungbuk, 712-714. ]
• Jang Won CHOI [ Dept. of Bioindustry, Daegu University, Kyungbuk, 712-714. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576