Ji Seon KIM, Yong Chang SEO, Woon Yong CHOI, Hyeon Yong LEE
언어
영어(ENG)
URL
https://www.earticle.net/Article/A144786
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원문정보
초록
영어
This study was to investigate the improvement of biological activities of Saposhnikovia divaricata through ultrasonification extraction process. Saposhnikovia divaricata was extracted at 60°C and 130 KHz for 30 min. The extraction yield was 26.1% (v/v) which was higher than that from convenational extraction using water at 100°C for 12 hours. All of extracts at a concentration of 1.0 mg/ml showed relatively low cytotoxicity on human normal kidney cell (HEK293) in range of 15∼ヲ25%. The inhibition ratios of several cancer cell lines such as human lung cancer cell A549, human gastric cancer cell AGS and human hepatocarcinoma cell Hep3B were measured using the sulforhodamine-B assay. The ultrasonification extracts of Saposhnikovia divaricata showed the highest cancer cell (A549, AGS, Hep3B) growth inhibition ratio as 77.3%, 79,1% and 75.9%, respectively. The immune B and T cell growth was improved by the ultrasonification extracts of Saposhnikovia divaricata up to 1.2×104 cells/mL and 1.0×104 cells/mL, respectively. The extract prepared also greatly increased the secretion of both IL-6 and TNF-α from ultrasonification process. This results can conclude that ultrasonification process effectively released active biomaterials which could important role in enhancing immune activity in the body.
Ji Seon KIM [ Medical & Bio-Material Research Center and Department of Biomaterials Engineering College of Bioscience and Biotechnology, Kangwon National University, Chuncheon 200-701. ]
Yong Chang SEO [ Medical & Bio-Material Research Center and Department of Biomaterials Engineering College of Bioscience and Biotechnology, Kangwon National University, Chuncheon 200-701. ]
Woon Yong CHOI [ Department of Biomaterials Engineering College of Bioscience and Biotechnology, Kangwon National University, Chuncheon 200-701. ]
Hyeon Yong LEE [ Medical & Bio-Material Research Center and Department of Biomaterials Engineering College of Bioscience and Biotechnology, Kangwon National University, Chuncheon 200-701. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동
간행물
간행물명
한국생물공학회 학술대회
간기
반년간
수록기간
1985~2013
십진분류
KDC 476DDC 576
이 권호 내 다른 논문 / 한국생물공학회 학술대회 2011년도 한국생물공학회 춘계학술발표대회