Earticle

초록

영어

A less frequently employed Escherichia coli strain W, yet possessing useful metabolic characteristics such as less acetic acid production and high L-valine tolerance, was metabolically engineered for the production of L-valine. The engineered E. coli W (ΔlacI Δ ilvA) strain overexpressing the ilvBNmut, ilvCED, ygaZH and lrp genes was able to produce an impressively high concentration of 60.7 g/L L-valine by fed-batch culture in 29.5 h, resulting in a high volumetric productivity of 2.06 g/L/h. The results obtained in this study suggest that E. coli W can be a good alternative to Corynebacterium glutamicum and E. coli K-12, which have so far been the most efficient L-valine producer. Furthermore, it is expected that various bioproducts including other amino acids might be more efficiently produced by this revisited platform strain of E. coli.[This work was supported by the Advenced Biomass R&D Center (ABC) of Korea Grant funded by the Ministry of Education, Science and Technology (2010-0029799). Further supports by the World Class University Program (R32-2008-000-10142-0) of the MEST were appreciated.]

키워드

저자

• Hye Min PARK [ Dept. of Chemical &Biomolecular Engineering, KAIST, Daejeon, 305-701. ]
• Jin Hwan PARK [ Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 program), BioProcess Engineering Research Center, KAIST, Daejeon, Korea, Center for Systems and Synthetic Biotechnology, Institute for the Biocentury, KAIST, Daejeon, Korea. ]
• Yu-Sin JANG [ Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 program), BioProcess Engineering Research Center, KAIST, Daejeon, Korea, Center for Systems and Synthetic Biotechnology, Institute for the Biocentury, KAIST, Daejeon, Korea. ]
• Jeong Wook LEE [ Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 program), BioProcess Engineering Research Center, KAIST, Daejeon, Korea, Center for Systems and Synthetic Biotechnology, Institute for the Biocentury, KAIST, Daejeon, Korea. ]
• Sang Yup LEE [ Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 program), BioProcess Engineering Research Center, KAIST, Daejeon, Korea, Center for Systems and Synthetic Biotechnology, Institute for the Biocentury, KAIST, Daejeon, Korea, Department of Bio and Brain Engineering and Bioinformatics Research Center, KAIST, Daejeon, Korea, Department of Biological Sciences, KAIST, Daejeon, Korea. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576