Earticle

현재 위치 Home

포스터 발표 : 유전자 및 대사공학

Systems Metabolic Engineering of Escherichia coli for the Efficient Production of Putrescine: A Four Carbon Diamine

첫 페이지 보기
  • 발행기관
    한국생물공학회 바로가기
  • 간행물
    한국생물공학회 학술대회 바로가기
  • 통권
    2011년도 한국생물공학회 춘계학술발표대회 (2011.04)바로가기
  • 페이지
    pp.196-196
  • 저자
    Hye Min PARK, Zhi-Gang QIAN, Xiao-Xia XIA, Sang Yup LEE
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A144776

※ 원문제공기관과의 협약기간이 종료되어 열람이 제한될 수 있습니다.

원문정보

초록

영어
Putrescine, also known as 1,4-diaminobutane, is an important nitrogen-containing platform chemical with numerous applications in industry. Particularly, putrescine is currently polycondensed with adipic acid to synthesize nylon-4,6 (Stanyl®, DSM), a superior engineering plastic because of its high melting point and mechanical strength as well as excellent solvent resistance. Current production of putrescine on industrial scale relies mainly on chemical synthesis from petrochemicals under harsh conditions. From an environmental point of view, the chemical synthesis route is undesirable.Here we show a sustainable bio-based process for putrescine production using metabolically engineered strain of Escherichia coli. The final engineered E. coli strain was able to produce 1.68 g L-1 of putrescine with a yield of 0.168 g per g glucose in a batch cultivation. Furthermore, high cell density cultivation allowed production of 24.2 g L-1 of putrescine within 32 hour. The strategy reported here should be useful for the bio-based production of putrescine from renewable resources, and also for the development of strains capable of producing other diamines, which are important as nitrogen-containing platform chemicals. [This work was supported by the Korean Systems Biology Research Project (20100002164) of the Ministry of Education, Science and Technology (MEST) through the National Research Foundation of Korea. Further support by the World Class University Program (R32-2008-000-10142-0) through the National Research Foundation of Korea funded by the MEST is appreciated.]

키워드

Diamine Putrescine Metabolic engineering

저자

  • Hye Min PARK [ Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical &Biomolecular Engineering (BK21 Program), BioProcess Engineering Research Center, Center for Systems and Synthetic Biotechnology, and Institute for the BioCentury, KAIST, 335 Gwahangno, Yuseong-gu, Daejeon 305-701, Republic of Korea ]
  • Zhi-Gang QIAN [ Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical &Biomolecular Engineering (BK21 Program), BioProcess Engineering Research Center, Center for Systems and Synthetic Biotechnology, and Institute for the BioCentury, KAIST, 335 Gwahangno, Yuseong-gu, Daejeon 305-701, Republic of Korea ]
  • Xiao-Xia XIA [ Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical &Biomolecular Engineering (BK21 Program), BioProcess Engineering Research Center, Center for Systems and Synthetic Biotechnology, and Institute for the BioCentury, KAIST, 335 Gwahangno, Yuseong-gu, Daejeon 305-701, Republic of Korea. ]
  • Sang Yup LEE [ Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical &Biomolecular Engineering (BK21Program), BioProcess Engineering Research Center, Center for Systems and Synthetic Biotechnology, and Institute for theBioCentury,Department of Bio and Brain Engineering, and Bioinformatics Research Center, Department of Biological Sciences KAIST, 335 Gwahangno, Yuseong-gu, Daejeon 305-701, Republic of Korea. ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
  • 설립연도
    1984
  • 분야
    공학>생물공학
  • 소개
    이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다 1. 생물공학 분야의 발전을 위한 연구 협력 2. 생물공학의 실용화를 촉진시키기 위한 산학 협동 3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최 4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간 5. 생물공학 발전을 위한 정책 건의 6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동

간행물

  • 간행물명
    한국생물공학회 학술대회
  • 간기
    반년간
  • 수록기간
    1985~2013
  • 십진분류
    KDC 476 DDC 576

이 권호 내 다른 논문 / 한국생물공학회 학술대회 2011년도 한국생물공학회 춘계학술발표대회

    피인용수 : 0건 (자료제공 : 네이버학술정보)

    함께 이용한 논문 이 논문을 다운로드한 분들이 이용한 다른 논문입니다.

      출처 : 네이버학술정보

        0개의 논문이 장바구니에 담겼습니다.

        페이지 저장
        소속기관 조회
        이용자님의 소속기관(단체)이 서비스에 가입되어 있는지 확인해 보십시오.
        기관회원에 소속되어 있는 이용자는 원문을 무료로 이용할 수 있습니다.