Earticle

초록

영어

Fumaric and malic acids commonly used chemicals and are closely associated with our everyday-life. A Gram-negative facultative capnophilic rumen bacterium, Mannheimia succiniciproducens, produces mainly succinic acid using anaplerotic pathway under anaerobic condition. M.succiniciproducens can’'t produce malate and fumarate in wild type strain. But M.succiniciproducens has a strong point for production of fumarate and malate production because it has strong anaplerotic pathway under CO2 condition. Recently, our group published papers about developing the high succinate production strain by gene knock-out. Based on this strain, The fumarate and malate production strain were constructed. Especially, When Fumarate reductase which converts fumarate to succinate was knock-out, this strain produce amount of malate and fumarate and do not produce succinate. The results provide useful information for the rational metabolic engineering by optimizing metabolic fluxes to improve fumarate and malate production in having a strong anaplerotic pathway strain such as rumen bacteria. [This work was supported by the Korean Systems Biology Research Project (20100002164) of the Ministry of Education, Science and Technology (MEST) through the National Research Foundation of Korea. Further support by the World Class University Program (R32-2008-000-10142-0) through the National Research Foundation of Korea funded by the MEST is appreciated.]

키워드

저자

• Hye Min PARK [ Metabolic and Biomolecular Engineering Laboratory, Department of Chemical and Biomolecular Engineering (BK21 program), BioProcess Engineering Research Center, and Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, KAIST, Daejeon, 305-701. ]
• Sol CHOI [ Metabolic and Biomolecular Engineering Laboratory, Department of Chemical and Biomolecular Engineering (BK21 program), BioProcess Engineering Research Center, and Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, KAIST, Daejeon, 305-701. ]
• Jeong Wook LEE [ Metabolic and Biomolecular Engineering Laboratory, Department of Chemical and Biomolecular Engineering (BK21 program), BioProcess Engineering Research Center, and Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, KAIST, Daejeon, 305-701. ]
• Sang Yup LEE [ Metabolic and Biomolecular Engineering Laboratory, Department of Chemical and Biomolecular Engineering (BK21 program), BioProcess Engineering Research Center, and Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, Department of Bio and Brain and Bioinformatics Research Center,KAIST, 335 Gwahangno, Yuseong-gu, Daejeon 305-701. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576