Earticle

초록

영어

Single nucleotide difference discrimination is important for field of genotyping including Single Nucleotide Polymorphism (SNP). Several technical approaches were applied for discriminating single nucleotide difference discrimination, ligation based technologies were broadly used which use DNA ligase and target specific probe set. Ligation based technology genotype the single nucleotide with ligation efficiency difference between perfectly matched probe and mismatched probe. If 3’' end of left probe oligo (LPO) hybridize perfectly with target site, LPO ligated with 5’' end of right probe oligo nucleotide (RPO). But in the case of mismatch between 3’' end of LPO and target site, ligation between LPO and RPO is still occurred in some case because of high reaction rate of DNA ligase, so it does not gives accurate information about genomic information of target site. We used commercially available alternative nucleobase such as 5’'-nitroindole, iso dC, iso dG in 5’' end of RPO to solve the problem by decreasing reaction rate of DNA ligase. By this approach, various matches and mismatch between 3’' end of LPO and target site was discriminated.

키워드

저자

• Hee Sung HWANG [ I-Bio program, Pohang University of Science and Technology. ]
• Gi Won SHIN [ I-Bio program, Pohang University of Science and Technology. ]
• Boram CHUNG [ I-Bio program, Pohang University of Science and Technology. ]
• Jeong Kyeong NA [ I-Bio program, Pohang University of Science and Technology. ]
• Seung Pil PACK [ Dept. of Biotechnology and Bioinformatics, Korea University, Jochiwon, Chungnam 339-700. ]
• Gyoo Yeol JUNG [ I-Bio program, Pohang University of Science and Technology. ]

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간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576