Earticle

초록

영어

Guanosine-5’diphosphate (GDP)-L-fucose is an essential precursor for the synthesis of fucosyloligosaccharides, which are involved in various biological and pathological functions such as cell adhesion, inflammation, tumor metastasis and host immune response modulation. In this study, key genes for GDP-Lfucose production, encoding GDP-D-mannose-4,6-dehydratase (GMD) and GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase (W caG) from Escherichia coli were introduced into C. glutamicum. GDP-L-fucose production was attempted by combinatorial overexpression of mannose-6-phosphate isomerase (manA), phosphomanno-mutase (manB) and GTP-mannose-1-phosphate guanylyl-transferase (manC) genes, which are also involved in GDP-D-mannose biosynthesis. Batch fermentations using the recombinant C. glutamicum systems showed that overexpression of gmd, wcaG, manB, manC was the most effective for GDP-L-fucose production and gave a 5.8-fold enhancement in specific GDP-L-fucose content compared with the control strain overexpressing GMD and WcaG only. A maximal GDP-L-fucose concentration of 86.2 mg/l was achieved in a batch fermentation of the recombinant C. glutamicum overexpressing GMD, WcaG, ManB and ManC.

키워드

저자

• Young-Wook CHIN [ Dept. of Agricultural Biotechnology, Seoul National University, Seoul, 151-742 ]
• Nam-Soo HAN [ Dept. of Food Science and Technology, Chungbuk National University, Cheongju, 361-763. ]
• Jin-Byung PARK [ Dept. of Food Science &Engineering, Ewha Womans University, Seoul, 120-750 ]
• Yong-Cheol PARK [ Dept. of Advanced Fermentation Fusion Science and Technology, Kookmin University, Seoul, 136-702. ]
• Jin-Ho SEO [ Dept. of Agricultural Biotechnology, Seoul National University, Seoul, 151-742. ]

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간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576