Earticle

초록

영어

Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293 is a lactic acid bacterium that converts pyruvate to L-(+)- and D-(-)-lactate by sterospecific enzymes designed as L-(+)- and D-(-)-lactate dehydrogenase (LDH), respectively. This study aims to determine the genes responsible for D-lactate formation in L. mesenteroides subsp. mesenteroides ATCC 8293, as well as to provide a basis for the realization of optically pure D-lactate production. We report the cloning and nucleotide sequence of the ldhD gene and LEUM_2043 which has not been annotated exactly from L. mesenteroides. The two genes had been cloned and expressed in Escherichia coli BL21(DE3) star with an inducible expression vector pETDuetTM-1 successfully. The two proteins, ldhD and LEUM_2043, had an apparent molecular mass of 36 kDa each on SDS-PAGE, and the LEUM_2043 was much more expression than ldhD. And the activity of ldhD and LEUM_2043 were similar. By now, the mechanism for D-lactate formation in L.mesenteroides has been characterized. Moreover, we extend our research to construct Leuconostoc strain which produces high level L-lactate and apply it as a starter culture.

키워드

저자

• LING LI [ Department of Food Science and Technology, Chungbuk National University, Cheongju, South Korea. ]
• Jung Mi PARK [ Department of Food Science and Technology, Chungbuk National University, Cheongju, South Korea. ]
• Hwayoung CHOI [ Department of Food Science and Technology, Chungbuk National University, Cheongju, South Korea. ]
• Ji Eun AHN [ Department of Food Science and Technology, Chungbuk National University, Cheongju, South Korea. ]
• Nam Soo HAN [ Department of Food Science and Technology, Chungbuk National University, Cheongju, South Korea. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576