Earticle

초록

영어

L. mesenteroides has been isolated in sugar refining process and it shows strong sucrose-dependant metabolic features such as dextran and mannitol production. In this study, a proteomic analysis was performed to understand the carbohydrate metabolism of this species in glucose medium and to uncover its regulatory changes in fructose or sucrose medium. For this, a LC-MS/MS was used to analyze the intracellular protein levels of L. mesenteroides ATCC 8293 when cultured in 2% glucose-, 2% sucrose-, or 1% glucose and 1% fructose-MRS media. Shotgun proteomic results obtained from duplicate samples showed high correlation and reproducibility. Totally 516 proteins were detected with high confidence in each cultures. When it growth in different carbon sources, L. mesenteroides ATCC 8293 preferentially used carbohydrate metabolism related pathway. In the central metabolic pathway, most of the proteins are consistently expressed. It showed that central metabolic pathway related proteins of L. mesenteroides ATCC 8293 have homeostasis. Also, L. mesenteroides ATCC 8293 have incomplete TCA cycle. When look at the expression level of that was not related with growth of L. mesenteroides ATCC 8293.

키워드

저자

• Eunyoung SEO [ Department of Food Science and Technology, Chungbuk National University, Cheongju 361-763. ]
• Yujin KIM [ Department of Food Science and Technology, Chungbuk National University, Cheongju 361-763. ]
• Jae-han KIM [ Department of Viticulture and Enology, University of California, Davis, CA 95616, USA. ]
• Nam Soo HAN [ Department of Food Science and Technology, Chungbuk National University, Cheongju 361-763. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576