Earticle

초록

영어

In agriculture, chitinolytic microorganisms have been widely used for biocontrol of fungal phytopathogens as cell wall of most fungal pathogens contains chitin to varying extent. Paenibacillus species are widely distributed in nature and have been reported for their biocontrol potential against bacteria and fungi owing to the production of cell wall degrading enzymes such as β-1,3-glucanases, cellulases, chitinases and proteases. This study indicated that plant growth promoting rhizobacterium P. polymyxa has higher chitinase activity compared to Bacillus thuringiensis which is the most widely used biopesticide and known primarily for its chitinase. A chitinase gene (chi45) from P. polymyxa was cloned, sequenced, and expressed in Escherichia coli. The gene consisted of an open reading frame of 1,248 nucleotides encoding a protein of 416 amino acids with a calculated molecular weight of 45,760 Da. Zymogram analysis indicated that the purified enzyme revealed the presence of the 45-kD chitinase. The purified chitinase was shown to inhibit the growth of Rhizoctonia solani, plant pathogenic fungus. Characterization of novel chitinase from P. polymyxa suggested that this enzyme is applicable for various industrial applications, including its use as biopesticide.

키워드

저자

• Ju JUNG [ School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Korea. ]
• Kyung Ok YU [ School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Korea. ]
• Ahmad Bazli RAMZI [ School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Korea. ]
• Young In KIM [ School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Korea. ]
• Sung Ok HAN [ School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Korea. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576