Earticle

초록

영어

Isopentenol is a natural alcohol and one of the most simple terpenoid. It is clear colorless oil that is widely used in cosmetic and pharmaceutical industry. Recently, ispentenol is produced as 5-carbon alcohols from the genetically modified E. coli, which are worthy alternative to present hydrocarbon fuels. But mass production of isopentenol is hindered due to its toxicity to host strain, E. coli. Thus, it is desirable to achieve an isopentenol tolerance of host strain through genetic engineering. In our study we have developed a robust method of random mutagenesis and selection for rapid evolution of tolerant strain. To enable this procedure, we constructed temperature-sensitive plasmids that temporarily increase the mutation frequency of their hosts by 3000 to 5,000-fold. Under appropriate selection pressure, microorganisms transformed with mutator plasmids can be quickly evolved to exhibit new, complex traits. Subsequently, the evolved strains were returned to their normal low mutation rate by curing the cells of the mutator plasmids to prevent the accumulation of undesirable spontaneous mutations. By using this approach, we were able to increase the tolerance of E. coli DH5α strain to isoepentenol up to 1.25g/liter. Our work demonstrated an efficient method for rapid strain improvement based on in vivo mutagenesis.This work was supported by a grant (NRF-2010-C1AAA001-0029084) from the Natonal Research Foundation and a grant from 21C Frontier Microbial Genomics and Application Center Program, MEST, and BK21 program of Korea

키워드

저자

• Asad Ali SHAH [ Division of Applied Life Science (BK21), PMBBRC, Gyeongsang National University, Jinju 600-701, Korea. ]
• Chonglong WANG [ Division of Applied Life Science (BK21), PMBBRC, Gyeongsang National University, Jinju 600-701, Korea. ]
• Sang-Hwal YOON [ Division of Applied Life Science (BK21), PMBBRC, Gyeongsang National University, Jinju 600-701, Korea. ]
• Jung-Hun KIM [ Division of Applied Life Science (BK21), PMBBRC, Gyeongsang National University, Jinju 600-701, Korea. ]
• Hui-Jeong JANG [ Division of Applied Life Science (BK21), PMBBRC, Gyeongsang National University, Jinju 600-701, Korea. ]
• Seo-Hee KANG [ Division of Applied Life Science (BK21), PMBBRC, Gyeongsang National University, Jinju 600-701, Korea. ]
• Eui-Sung CHOI [ Systems Microbiology Research Center, KRIBB, Daejeon 305-806, Korea ]
• Seon-Won KIM [ Division of Applied Life Science (BK21), PMBBRC, Gyeongsang National University, Jinju 600-701, Korea. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576