Efficient Production of 1,3-propandiol from Glycerol by Constitutive Expression of 1,3-propandiol Oxidoreductase Gene in the Engineered Klebsiella pneumoniae to Eliminate by-product Formation
Baek-rock OH, Jeong-Woo SEO, Heo-Yeon HEO, Don-Hee PARK
언어
영어(ENG)
URL
https://www.earticle.net/Article/A144681
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원문정보
초록
영어
In this study, we developed an efficient process for 1,3-PD production from glycerol by genetic engineering of K. pneumoniae AK mutant strains, in which by-products formation was eliminated but IPTG induction was necessary for maximal production of 1,3-PD. A series of recombinant strain was designed to constitutively express dhaB and/or dhaT gene using bacteriophage T5 PDE20 and rho-independent transcription termination signal of Rahnella aquatilis levansurcrase gene. Among them, AK/pConT expressing dhaT alone was the most proper for 1,3-PD production. Fed-batch fermentation showed efficient production of 1,3-PD from both pure or crude glycerol without by-products formation. Further engineering to enhance production level would provide an economical biological process for 1,3-PD production.
Baek-rock OH [ Microbe-based Fusion Technology Research Center, Jeonbuk Branch Institute, KRIBB, Jeongeup Jeonbuk 580-185, Korea, Interdisciplinary Program of Graduate School for Bioenergy &Biomaterials, Chonnam National University, Gwangju 500-757, Korea. ]
Jeong-Woo SEO [ Microbe-based Fusion Technology Research Center, Jeonbuk Branch Institute KRIBB, Jeongeup Jeonbuk 580-185, Korea. ]
Heo-Yeon HEO [ Microbe-based Fusion Technology Research Center, Jeonbuk Branch Institute, KRIBB, Jeongeup Jeonbuk 580-185, Korea. ]
Don-Hee PARK [ Interdisciplinary Program of Graduate School for Bioenergy &Biomaterials, Chonnam National University, Gwangju 500-757, Korea. 3Radiation Research Division for Biotechnology, Korea Atomic Energy Research Institute, Jeonbuk 580-185, South Korea. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동
간행물
간행물명
한국생물공학회 학술대회
간기
반년간
수록기간
1985~2013
십진분류
KDC 476DDC 576
이 권호 내 다른 논문 / 한국생물공학회 학술대회 2011년도 한국생물공학회 춘계학술발표대회