Recently small regulatory RNAs have been discovered in prokaryotic cells to regulate intracellular processes. They repress translation by preventing ribosome binding to mRNA. The regulatory small RNAs could provide a new level of regulation in synthetic biology to control synthetic circuits in concert with transcriptional regulations. We developed synthetic small regulatory RNAs repressing the translation of DsRed2 mRNA and also constructed three different sRNAs for the mRNAs of LuxR, AraC, and KanR without cross-reactivity. The results suggest that gene expression can be fine-tuned by designed artificial small RNAs. The possibility of designing small regulatory RNAs may facilitate the development of precisely regulated synthetic circuits. [This work was supported by the Korean Systems Biology Research Project (20100002164) of the Ministry of Education, Science and Technology (MEST) through the National Research Foundation of Korea. Further support by the World Class University Program (R32-2008-000-10142-0) through the National Research Foundation of Korea funded by the MEST is appreciated.]
키워드
small regulatory RNAsynthetic biologymetabolic engineeringsynthetic RNA
저자
Dokyun NA [ Department of Chemical and Biomolecular Engineering, KAIST. ]
Seung Min YOO [ Department of Chemical and Biomolecular Engineering, KAIST ]
Sang Yup LEE [ Department of Chemical and Biomolecular Engineering (BK21 program), BioProcess Engineering Research Center, Bioinformatics Research Center, and Institute for the BioCentury, KAIST, Republic of Korea; Department of Bio and Brain Engineering, KAIST, Republic of Korea. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동
간행물
간행물명
한국생물공학회 학술대회
간기
반년간
수록기간
1985~2013
십진분류
KDC 476DDC 576
이 권호 내 다른 논문 / 한국생물공학회 학술대회 2011년도 한국생물공학회 춘계학술발표대회