Translation, process that synthesizes the protein, is related with the cell growth because proteins which synthesized through translation play the major role in the biological system. The modulation of translation elongation rate could affect protein synthesis rate and change the growth rate. Among the translation elongation factors, EF-G is a key factor in translation elongation since it determines how fast translocation of ribosome occurs. To modulate the growth rate, a genetic circuit was constructed and applied to on/off switch. fusA gene coding EF-G is regulated by lambda promoter which is repressed by cI repressor which is expressed by IPTG induction. The regulation system of fusA by cI repressor was validated by measuring the growth rate of BL21 (DE3) deleted chromosomal fusA in the various IPTG induction time and concentration. In this research, growth rate was decreased after 2hr from IPTG induction generally and more decreased as IPTG concentration was increased. It is shown that this genetic circuit could control the growth of cell.
키워드
TranslationEF-GGenetic circuit
저자
Byung Eun MIN [ School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology, Pohang, Gyeongbuk, 790-784. ]
Sang Woo SEO [ Department of Chemical Engineering, Pohang University of Science and Technology, Pohang, Gyeongbuk, 790-784. ]
Gyoo Yeol JUNG [ School of Interdisciplinary Bioscience and Bioengineering and Dpartment of Chemical Engineering, Pohang University of Science and Technology, Pohang, Gyeongbuk, 790-784. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동
간행물
간행물명
한국생물공학회 학술대회
간기
반년간
수록기간
1985~2013
십진분류
KDC 476DDC 576
이 권호 내 다른 논문 / 한국생물공학회 학술대회 2011년도 한국생물공학회 춘계학술발표대회