Bombyx mori genome has three ferritins, iron storage proteins, Fer1HCH, Fer2LCH, and Fer3HCH. Of them, only Fer2LCH mRNA was found in our EST library constructed from the day 1 of silkworm eggs and had a putative N-glycosylation site. Temporal, spatial, and inducible expression analyses during embryogenesis revealed that the silkworm Fer2LCH mRNA was abundant from 6 h to 6 days after oviposition. Transcription of Fer2LCH was not affected by immune challenge, oxidative, and iron stress. To understand the difference in folding and assembly of recombinant protein secreted in insect cell or silkworm, Fer2LCH purified in both production systems was analyzed. The Fer2LCH proteins produced by both systems were N-glycosylated but not O-glycosylated. Lectin blot analysis indicated that only Fer2LCH produced by silkworm was terminated by mannose, GlcNAc, and Fucose residues. The association of ERp57 with Fer2LCH glycoprotein from hemolymph was stronger than with it from cell, and the interaction with PDI, Bip, calnexin, and calreticulin were very similar in both systems. Our data suggest that Fer2LCHs synthesized in cells and silkworm interact probably differently with the ER chaperones.
키워드
recombinat proteiglycosylationER chaperone
저자
Sun Mee Hong [ Department of Research and Development, Gyeongbuk Institute for Marine Bioindustry (GIMB) Laboratory of Silkworm Sciences, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences, Japan. ]
Jae Man Lee [ Laboratory of Silkworm Sciences, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences, Japan. ]
Takahiro Kusakabe [ Laboratory of Silkworm Sciences, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences, Japan. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동
간행물
간행물명
한국생물공학회 학술대회
간기
반년간
수록기간
1985~2013
십진분류
KDC 476DDC 576
이 권호 내 다른 논문 / 한국생물공학회 학술대회 2011년도 한국생물공학회 춘계학술발표대회