Jun-hee Lee, Sung-jun Yang, Sung-mo Joo, So-ra Choi, Hyun-seo Kim, Byoung-Oh Hwang, Nam-il Kim, Han-oh Park
언어
영어(ENG)
URL
https://www.earticle.net/Article/A144657
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원문정보
초록
영어
There has recently been much research conducted on the high temperature activity and thermal stability of reverse transcriptases. Traditional M-MLV reverse transcriptase possesses low thermal stability, allowing for reverse transcription reactions to occur only at relatively low temperatures (42℃). This limitation prevents the efficient transcription of RNA molecules with complex secondary structures. To resolve this, BIoneer has utilized synthetic biotechnology and protein engineering to develop a thermostable reverse transcriptase with activity at high temperatures of 50 °C and above, and dubbed the enzyme RocketScript. Higher temperature reverse transcription reactions allow optimization of cDNA synthesis experiments with temperature. RocketScript is Bioneer’ exclusive M-MLV-based thermostable reverse transcriptase (RTase). Because the original M-MLV RTase has maximum activity at relatively low temperatures (42 °C), it caused several problems during reverse transcription of complex secondary structure RNA species. RocketScript has thermostable activity (42~70°C), allowing efficient cDNA synthesis from complex secondary structure RNA.
저자
Jun-hee Lee [ Genetic Science Division,Bioneer Corporation, 49-3,Munpyung-dong, Daedeok-Gu, Daejeon, Republic of Korea ]
Sung-jun Yang [ Genetic Science Division,Bioneer Corporation, 49-3,Munpyung-dong, Daedeok-Gu, Daejeon, Republic of Korea ]
Sung-mo Joo [ Genetic Science Division,Bioneer Corporation, 49-3,Munpyung-dong, Daedeok-Gu, Daejeon, Republic of Korea ]
So-ra Choi [ Genetic Science Division,Bioneer Corporation, 49-3,Munpyung-dong, Daedeok-Gu, Daejeon, Republic of Korea ]
Hyun-seo Kim [ Genetic Science Division,Bioneer Corporation, 49-3,Munpyung-dong, Daedeok-Gu, Daejeon, Republic of Korea ]
Byoung-Oh Hwang [ Genetic Science Division,Bioneer Corporation, 49-3,Munpyung-dong, Daedeok-Gu, Daejeon, Republic of Korea ]
Nam-il Kim [ Genetic Science Division,Bioneer Corporation, 49-3,Munpyung-dong, Daedeok-Gu, Daejeon, Republic of Korea ]
Han-oh Park [ Genetic Science Division,Bioneer Corporation, 49-3,Munpyung-dong, Daedeok-Gu, Daejeon, Republic of Korea ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동
간행물
간행물명
한국생물공학회 학술대회
간기
반년간
수록기간
1985~2013
십진분류
KDC 476DDC 576
이 권호 내 다른 논문 / 한국생물공학회 학술대회 2011년도 한국생물공학회 춘계학술발표대회