Proteases, playing an important role in a wide variety of physiological processes, are enzymes that selectively cleave peptide bonds in proteins and polypeptides. Because of these relationships between proteases and diseases, the need for synthesis fast, sensitive, and specific protease assay materials has increased considerably in the last few years. Dityrosine (DY), which is an unusual amino acid formed as a result of normal posttranslational processing. Many researchers have used dityrosine as an important biomarker for oxidatively modified proteins because of its fluorescence and stability. However, in this study we try to use dityrosine for another area such as protease assay. Therefore, we synthesized DBDY-(INH)2 as a simple protease assay material and experimented on assay with this material. The results of this experiment show that DBDY-(INH)2 can be used as a specific and sensitive assay material about papain.
키워드
DityrosineFluorescenceProtease AssayPapain
저자
Chan-Jin KIM [ Department of Chemical and Biomolecular Engineering, Yonsei University, Seoul, 120-749. ]
Dong-Ik LEE [ Department of Chemical and Biomolecular Engineering, Yonsei University, Seoul, 120-749. ]
Ik-Sung AHN [ Department of Chemical and Biomolecular Engineering, Yonsei University, Seoul, 120-749. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동
간행물
간행물명
한국생물공학회 학술대회
간기
반년간
수록기간
1985~2013
십진분류
KDC 476DDC 576
이 권호 내 다른 논문 / 한국생물공학회 학술대회 2011년도 한국생물공학회 춘계학술발표대회