Earticle

초록

영어

A novel lipolytic enzyme was isolated from a metagenomic library after demonstration of lipolytic activity on an LB agar plate containing 1% (w/v) tributyrin. A novel esterase gene (estIM1), encoding a lipolytic enzyme (EstIM1), was cloned using a shotgun method from a pFosEstIM1 clone of the metagenomic library, and the enzyme was characterized. The deduced amino acid sequence was 62% identical to that of an esterase from an uncultured bacterium (ABQ11271). EstIM1 was active over a temperature range of 1-50ºC, at alkaline pH. The activation energy for hydrolysis of p-nitrophenyl propionate was 1.04 kcal/mol, within a temperature range of 1-40ºC. The activity of EstIM1 was about 60% of maximal even at 1ºC, suggesting that EstIM1 is efficiently cold-adapted. Further characterization indicated that the esterase may be very valuable in industrial applications. (This work was supported by a HTS-based Integrated Technology Development grant and by Basic Science Research Program from the MEST.)

키워드

저자

• Kyong-Cheol KO [ Microbe-based Fusion Technology Research Center, KRIBB, 1404 Sinjeong-dong, Jeongeup, Jeonbuk 580-185. ]
• Soon-Ok RIM [ Microbe-based Fusion Technology Research Center, KRIBB, 1404 Sinjeong-dong, Jeongeup, Jeonbuk 580-185. ]
• Bong Seok SHIN [ Microbe-based Fusion Technology Research Center, KRIBB, 1404 Sinjeong-dong, Jeongeup, Jeonbuk 580-185. ]
• Geun-Joong KIM [ Microbe-based Fusion Technology Research Center, KRIBB, 1404 Sinjeong-dong, Jeongeup, Jeonbuk 580-185. ]
• Jae Jun SONG [ Microbe-based Fusion Technology Research Center, KRIBB, 1404 Sinjeong-dong, Jeongeup, Jeonbuk 580-185. ]
• Jong Hyun CHOI [ Microbe-based Fusion Technology Research Center, KRIBB, 1404 Sinjeong-dong, Jeongeup, Jeonbuk 580-185. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576