Earticle

초록

영어

To explore the effects of the ionic liquids on enzymatic catalyses, the horseradish peroxidase catalyzed oxidation of 2-methoxyphenol with H2O2 was studied in the aqueous mixtures of the three ionic liquids, 1-butyl-3-methylimidazolium tetrafluoroborate ([BMIM][BF4]), N-butyl-3- methypyridinium tetrafluoroborate ([BMPy][BF4]), and 1-butyl-3- methylimidazolium methylsulfate ([BMIM][MeSO4]). [BMIM][BF4] and [BMPy][BF4] share an anion (BF4 -) while [BMIM][BF4] and [BMIM][MeSO4] have a cation (BMIM+) in common. On the addition of [BMIM][BF4] or [BMPy][BF4], Km increased while kcat decreased. In the aqueous mixtures of [BMIM][ MeSO4], however, both Km and kcat decreased. Equilibrium partitioning of the substrate with n-octanol as a standard solvent revealed that the three ionic liquids stabilize the free substrate resulting in the diminished binding affinity of the substrate onto the enzyme. Based on these kinetics and thermodynamics investigations, anionic parts of the ionic liquids are presumed to exert a major role to determine how the enzymatic catalysis is affected by the ionic liquids; BF4 - inhibits the enzyme non-competitively, but MeSO4 - uncompetitively.

키워드

저자

• Keungarp RYU [ Dept Chem Engr Bioengr, University of Ulsan, Ulsan 680-749. ]
• Junghee PARK [ Dept Chem Engr Bioengr, University of Ulsan, Ulsan 680-749. ]
• Haksung LEE [ Dept Chem Engr Bioengr, University of Ulsan, Ulsan 680-749. ]

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간행물

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• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576