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Immobilization and Stabilization of Lipase in the form of Enzyme Precipitation Coatings on Polymer Nanofibers

첫 페이지 보기
  • 발행기관
    한국생물공학회 바로가기
  • 간행물
    한국생물공학회 학술대회 바로가기
  • 통권
    2011년도 한국생물공학회 춘계학술발표대회 (2011.04)바로가기
  • 페이지
    pp.177-177
  • 저자
    Ji-Young EOM, Hyo Jin AN, Hye-Jin LEE, Seung-Hyun JUN, Sang Youn HWANG, Min-Kyu OH, Jungbae KIM
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A144644

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원문정보

초록

영어
Lipase (LP) was immobilized and stabilized in the form of enzyme precipitate coatings (EPCs) on electrospun and ethanol-dispersed polystyrene-poly (styrene-co-maleic anhydride) (PS-PSMA)nanofibers (EtOH-NF). LP precipitate coatings (EPCs-LP) were prepared in a three-step process, consisting of covalent attachment, LP precipitation, and cross-linking of precipitated LPs onto the covalently attached LPs via glutaraldehyde treatment. EPCs-LP improved the LP activity and stability when compared to covalently attached LPs (CA-LP) and the enzyme coatings of LPs (EC-LP) without the LP precipitation. For example, the use of 40% (w/v) ammonium sulfate resulted in EPC40-LP with the highest activity, which was 4.0 and 3.6 times higher than those of CA-LP and EC-LP, respectively. After 165-day incubation under rigorous shaking at 200 rpm, the residual activities of EPC50-LP were 0.5 μ M/min per mg of EtOH-NF, representing 113 and 75 times higher than those of CA-LP and EC-LP, respectively. When LP was partially purified via a simple ammonium sulfate precipitation and dialysis, both activities and stabilities of EC-LP and EPC-LP could be marginally improved. It is anticipated that the improved LP activity and stability in the form of EPCs would allow for their potential applications in various bioconversion processes such as biodiesel production and ibuprofen resolution.

키워드

Lipase Enzyme precipitate coatings Electrospun polymer nanofibers Enzyme stabilization

저자

  • Ji-Young EOM [ Department of Chemical and Biological Engineering, Korea University, Seoul 136-701. ]
  • Hyo Jin AN [ Department of Chemical and Biological Engineering, Korea University, Seoul 136-701. ]
  • Hye-Jin LEE [ Department of Chemical and Biological Engineering, Korea University, Seoul 136-701. ]
  • Seung-Hyun JUN [ Department of Chemical and Biological Engineering, Korea University, Seoul 136-701. ]
  • Sang Youn HWANG [ Department of Chemical and Biological Engineering, Korea University, Seoul 136-701. ]
  • Min-Kyu OH [ Department of Chemical and Biological Engineering, Korea University, Seoul 136-701. ]
  • Jungbae KIM [ Department of Chemical and Biological Engineering, Korea University, Seoul 136-701. ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
  • 설립연도
    1984
  • 분야
    공학>생물공학
  • 소개
    이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다 1. 생물공학 분야의 발전을 위한 연구 협력 2. 생물공학의 실용화를 촉진시키기 위한 산학 협동 3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최 4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간 5. 생물공학 발전을 위한 정책 건의 6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동

간행물

  • 간행물명
    한국생물공학회 학술대회
  • 간기
    반년간
  • 수록기간
    1985~2013
  • 십진분류
    KDC 476 DDC 576

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