Earticle

초록

영어

The stabilization of the proteins by various techniques is acquiring significance because of its applications in various fields of biotechnology. Over the years, different stabilization protocols as well as novel strategies were evolved for stabilizing the proteins. These stabilization offers over a vast number of applications in medical and industrially important proteins. A method to achieve the stabilization of the proteins is cyclization of the polypeptide back bone and it has been shown that cyclization of peptides frequently improves their stability and biological activity. Protein splicing mechanism provided the novel tools to cyclize the back bone of the desired protein. Inteins are the protein elements join the two ends by catalyzing the chemical steps acyl migration, leading to the formation of a thioester, forms the peptide bond and cyclize the protein. However, the cyclization process will not occur to complete extent either invitro or invivo leading to the mixture of both linear and cyclic proteins which are difficult to selectively separate. In the present investigation the efforts were made to study the process of cyclisation in vitro and in vivo using the well studied protein GFP (Green Fluorescent Protein). In the study various constructs of EGFP protein having variable numbers of HIS tags at N and C terminals express along with inteins to make it cyclize and selective purification of stabilized cyclic EGFP.

키워드

저자

• Pamidimarri D V N SUDHEER [ Dept. of Chemical and Biochemical Engineering, Dongguk University-Seoul, Seoul, 100-715. ]
• Taek Jin KANG [ Dept. of Chemical and Biochemical Engineering, Dongguk University-Seoul, Seoul, 100-715. ]

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간행물

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• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576