Earticle

초록

영어

A Thermococcus onnurineus NA1 has been studied for growth and hydrogen (H2) production from fomate and carbon monoxide (CO) as a main carbon source. The effect of amino acid and flue gas on growth and H2 production was examined in MM1 medium. And stability of H2 production experiment was employed. In addition, H2 production under resting cell conditions in different pH and temperature was investigated. The maximum specific growth rates in formate and CO were found to be 0.33 h-1 and 0.30 h-1, respectively. The addition of three amino acids (leucine, isoleucine and valine) to formate MM1 medium, showed an increase in maximum specific growth rate to 0.50 h-1 and final cell density was increase double. Among flue gas, only CO2 shows a little inhibition on initial growth and H2 production. The stability of H2 production activity with formate (100mM) and CO (100%, head space) has been study in 4 days. There was no decrease H2 production. Under resting cell conditions, optimum pH was around 6 to 7 and temperature was 80℃. NA1 produced H2 by water-gas shift reaction from CO with a theoretical yield of 1(mol/mol). T. onnurineus NA1 has good potential to be used for H2 production given the fact that H2 productivity of 20 mmol/mg/h. NA1 cells have the capability for repeated H2 production giving it an edge for commercial water-gas shift reactions.

키워드

저자

• Jungeun LEE [ Dept. of Chemical and Biomolecular Engineering, Pusan National University. ]
• Gurpreet KAUR [ Dept. of Chemical and Biomolecular Engineering, Pusan National University. ]
• Eunhee SEOL [ Dept. of Chemical and Biomolecular Engineering, Pusan National University. ]
• Sunghoon PARK [ Dept. of Chemical and Biomolecular Engineering, Pusan National University. ]

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발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576