One Streptomyces strain, which was recently isolated from Korean soil, produced extracellular lipase in the OSYM culture medium. The enzyme was purified using a single step gel permeation chromatography on Sepharose CL-6B. The enzyme was nearly homogenous in SDS-PAGE analysis with a single band. The activity was optimum at 40 °C and pH 7.0 and was stable between pH 5.0 and 8.0 and below 50 °C. Most of the tested metal ions as well as detergents showed inhibitory effect on the enzyme activity, but with various extents.. The enzyme showed highest hydrolytic activity with p-nitrophenyl palmitate (pNPP), a long chain substrate and Km and Vmax of the enzyme for the substrate were determined to be 0.24 mM and 4.6 mM/min mg, respectively. Furthermore, the enzyme hydrolyzed triolein producing 1,2- and 1,3-diolein along with oleic acid. More importantly, it catalyzed biodiesel production in presence of methanol and various oils; therefore, it can be applicable in bio-energy industry.
키워드
StreptomyceslipaseBioenergy
저자
Seung Sik CHO [ Department of Pharmacy, College of Pharmacy, Chosun University, Gwangju 501-759, Korea. ]
Da Jeong PARK [ Department of Pharmacy, College of Pharmacy, Chosun University, Gwangju 501-759, Korea. ]
Jin Cheol YOO [ Department of Pharmacy, College of Pharmacy, Chosun University, Gwangju 501-759, Korea. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동
간행물
간행물명
한국생물공학회 학술대회
간기
반년간
수록기간
1985~2013
십진분류
KDC 476DDC 576
이 권호 내 다른 논문 / 한국생물공학회 학술대회 2011년도 한국생물공학회 춘계학술발표대회