The actinomycetes strain J392 was found to exhibit broad spectrum of antimicrobial activity towards various pathogenic microorganism. The 16S rRNA sequence revealed that the strain was closely related to Streptomyces lanatus with 98.2% homology. Based on its genetic, morphological and biochemical characteristic the strain J392 was confirmed as belonging to the genus Streptomyces and we named as Streptomyces sp. J392. The production of antibiotic from this strain was found to be most favorable when cultured for 2 days at 28°C and 180rpm in shaking incubator, using glucose and peptone as respective carbon and nitrogen sources. The antibiotic was extracted using ethyl acetate (1:1 v/v) and partially purified using both column and thin layer chromatography techniques. Two antibiotic compounds based on its different solubility on ethyl acetate and methanol, were identified. Antimicrobial activity of the compound against different pathogenic strains was assessed using the minimum inhibitory concentrations (MICs). It was found to be active against different strains, including MRSA 693E, MRSA (4-5), MRSA (5-3), MRSA (5-3), VRSA, Enterococcus faecalis (ATCC 29212), Bacillus subtilis (ATCC 1928), Staphylococcus aureus (KCTC 1928) and Mycobacterium smegmatis (ATCC 9341). These result suggest that J392s possessing potential antibacterial agent.
키워드
Antimicrobial CompoundsIsolationPurification
저자
Seung KIM [ Department of Alternative Medicine, College of Health and Welfare and Education, Gwangju University, Gwangju 503-703, Korea. ]
Hyo Jeong LEE [ Department of Alternative Medicine, College of Health and Welfare and Education, Gwangju University, Gwangju 503-703, Korea. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동
간행물
간행물명
한국생물공학회 학술대회
간기
반년간
수록기간
1985~2013
십진분류
KDC 476DDC 576
이 권호 내 다른 논문 / 한국생물공학회 학술대회 2011년도 한국생물공학회 춘계학술발표대회