Conversion of cellulose to glucose is the most critical step for utilization of cellulose as biomass. Enzymatic hydrolysis of cellulose is achieved by cooperation of three types of cellulases including endoglucanase, exoglucanase, and β-glucosidase. Recently, yeast strains which are engineered to express cellulases have been developed to improve bioethanol production from lignocellulosic biomass. In this study, we focused on searching for endoglucanases that possess sufficient activity when expressed on yeast surface. We expressed various endoglucanases from fungi and bacteria on the surface of Sacchoromyces cerevisiae, and the activities of these recombinant enzymes were determined at various temperature conditions using Carboxymethylcellulose (CMC). As a result, Clostridium thermocellum CelD and Thermoascus aurantiacus endoglucanase I showed enzyme activities comparable with that of Trichoderma reesei endoglucanase II, which is widely used for bioethanol production studies. Therefore, these novel endoglucanases might be useful to generate yeast strains for consolidate bioprocessing of lignocellulosic biomass to ethanol.
키워드
yeastendoglucanaseyeast surface display
저자
Seung-Ho BAEK [ School of Chemical and Biological Engineerin, Seoul National University. ]
Ji-Sook Hahn [ School of Chemical and Biological Engineerin, Seoul National University. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동
간행물
간행물명
한국생물공학회 학술대회
간기
반년간
수록기간
1985~2013
십진분류
KDC 476DDC 576
이 권호 내 다른 논문 / 한국생물공학회 학술대회 2011년도 한국생물공학회 춘계학술발표대회