Earticle

초록

영어

To produce a novel bacteriocin against fish pathogenic bacteria, Lactococcus lactis subsp. was isolated from flounders collected from aquaculture farms. The antimicrobial activity and some physico-chemical properties of the purified bacteriocin were studied. The bacteriocin was purified by 15% PEG 4000/20% Na2SO4 and ethanol saturation followed by twice C-18 reverse phase HPLC. The molecular weight of the bacteriocin measured by MALDI-TOF was 3.3 kDa. We found that the bacteriocin inhibited the growth of gram-positive bacteria including fish pathogenic bacteria, Streptococcus sp. and Lactococcus garviae, but not the growth of gram-negative bacteria. Especially, S. pleomorphus indicator strain (400 arbitrary units ml-1), which is a harmful pathogenic bacteria to aquaculture fish, was shown to be most sensitive . The bacteriocin was found to be stable at 100°C for 20 min and in pH ranged from 3.0-4.0, but it was unstable at pH 10.0. The results indicates that antimicrobial agent produced by L. lactis subsp. may be a good candidate instead of antibiotics to apply in aquaculture and seafood industries.

키워드

저자

• Won-Seok HEO [ Dept. of Biotechnology, Pukyong National University, Busan, 608-737. ]
• Eun-Young KIM [ Dept. of Biotechnology, Pukyong National University, Busan, 608-737. ]
• Yu-Ri KIM [ Dept. of Biotechnology, Pukyong National University, Busan, 608-737. ]
• In-Soo KONG [ Dept. of Biotechnology, Pukyong National University, Busan, 608-737. ]

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간행물

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• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576