To breed optimal yeast strain for bioethanol production, we planed a strategy for breeding yeast harboring various industrially useful characters, such as ethanol tolerance, thermotolerance and β-1,3-glucanase activity. In this study, a BY4742Δexg1/pAInu-exgA strain which has extracellular β -1,3-glucanase activity from Aspergillus oryzae and YKY020 strain which has phenotype of ethanol tolerance and thermothlerance, were fused by yeast protoplast fusion. By cell fusion, 12 fusion cells were selected and six cells (No. 3, 9, 10, 11, 12 and 13) among 12 cells grew well compared with YKY020 and BY4742Δexg1/pAInu-exgA strains in thermotolerance test at 40℃. Subsequently, in ethanol tolerance (7% ethanol concentration) and β-1,3-glucanase activity tests, the fusion cell No. 11 showed enhanced characters compared with YKY020 and BY4742Δexg1/pAInu-exgA strain. It was proved that new yeast strain is easily breeded by fusion among yeasts having different characters and same mating type. This fusion cell called BYK-F11 strain, and this strain will be further improved by several times protoplast fusion to use bioethanol production or industrial process.
A Hwang PARK [ Department of Biomaterial Control, Dong-Eui University, Busan 614-714, Korea. ]
Min Ji KIM [ Department of Biotechnology and Bioengineering, Dong-Eui University, Busan 614-714, Korea. ]
Yeon Hee KIM [ Department of Biomaterial Control, Dong-Eui University, Busan 614-714, Korea, Department of Biotechnology and Bioengineering, Dong-Eui University, Busan 614-714, Korea. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동
간행물
간행물명
한국생물공학회 학술대회
간기
반년간
수록기간
1985~2013
십진분류
KDC 476DDC 576
이 권호 내 다른 논문 / 한국생물공학회 학술대회 2011년도 한국생물공학회 춘계학술발표대회