Earticle

초록

영어

In the bioindustry, it has been large category in that raw materials convert into value-added products by fermentation process. The efficient strain improvement is necessary to provide cost-competitiveness in the biotechnological production of commodity chemical. Although many techniques are available for generating improved strain in library, there is still limiting step, separating improved strain during combinatorial approach. To improve strain as high lysine producer, an artificial enrichment system has been constructed for efficient strain separation in combinatorial approach. The artificial enrichment system is composed of lysine riboswitch and dual selectable selection marker. The riboswitch responding lysine concentration regulated downstream gene expression. The operation of riboswitch in the enrichment tool was detected with fluorescence change by sgfp, reporter gene, expression level change. After that, the downstream gene has been replaced with TetA, dual selection marker. The expression level change caused of lysine concentration change was detected by specific growth rate change. Therefore, with appropriate selection pressure high lysine producer was enriched. This artificial enrichments system can be applied to further combinatorial approach.

키워드

저자

• Jina YANG [ Department of Chemical Engineering ]
• Sang woo SEO [ Department of Chemical Engineering ]
• Gyoo Yeol JUNG [ Department of Chemical Engineering and School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology, San 31, Hyoja-dong, Nam-gu, Pohang, Gyeongbuk, Korea, 790-784. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576