Earticle

초록

영어

Rapamycin, macrocyclic polyketide produced by Streptomyces hygroscopicus, is one of the potent immunosuppressants. In this study, classical mutation, genome shuffling and gene manipulation have been carried out to improve the rapamycin production. In addition, small scale culture and bioassay condition were developed for enhancing the screening efficiency of rapamycin hyper-producing mutants. A mutant Ra-II-111-01, which has 100% higher productivity than that of the parent strain Ra-I-88-3, was obtained by UV mutation under malonic acid and propionate as selective agent. When an additional copy of rapH gene, one of the positive regulatory gene of rapamycin biosynthesis, was inserted in Ra-I-88-3 strain, rapamycin production was increased by 17% on average. The best condition of protoplast formation for genome shuffling was determined by optimizing the culture media composition and lysozyme treatment condition. By optimizing the smallscale culture condition, it was observed that rapamycin productivity in 24 and 96 square deep well plate cultivation was 100% and 40% of that in flask scale, respectively.

키워드

저자

• Yoo-Won LEE [ Fermentation Research Laboratory, CKDBiO Research Institute, Ansan 425-100, Korea. ]
• Seong-Sun YU [ Fermentation Research Laboratory, CKDBiO Research Institute, Ansan 425-100, Korea. ]
• Young Ji YOO [ Department of Chemistry and Nano Science, Ewha Women University, Seoul 120-750, Korea. ]
• Yeo Joon YOON [ Department of Chemistry and Nano Science, Ewha Women University Seoul 120-750, Korea. ]
• Tae Won KANG [ Fermentation Research Laboratory, CKDBiO Research Institute, Ansan 425-100, Korea. ]
• Heui-Il KANG [ Fermentation Research Laboratory, CKDBiO Research Institute, Ansan 425-100, Korea. ]
• Jang-Woo PARK [ Fermentation Research Laboratory, CKDBiO Research Institute, Ansan 425-100, Korea. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576