Earticle

초록

영어

Galactosemia, one of the major newborn metabolic disorders, is caused by genetic enzyme deficiencies of galactose metabolism leading to the accumulation of galactose and galactose 1-phosphate in blood.1 Therefore, the diagnosis of galactosemia is generally achieved by measuring galactose or galactose-1-phosphate concentration using clinical dried blood spot specimens which adsorb a trace amount of blood. Up to now, several screening methods have been available such as biological inhibition assays developed by Guthrie and Paigen, high performance liquid chromatography and enzymatic colorimetric assay to determine galactose concentration.2,-4 However, these methods often require labor-intensive and time-consuming procedures or complicated and expensive instrumentation. To overcome these limitations, herein, we report a new galactose assay by utilizing two bioluminescent Escherichia coli immobilized within agarose gel arrayed on a well plate. galT knockout strain (galT-) was genetically engineered to inhibit the cell growth in the presence of galactose in a sample. A relative luminescence unit (RLU) value corresponding to the galT- cell growth induced by glucose was subtracted from the RLU value corresponding to the normal cell growth induced by both glucose and galactose. From the differences of RLU values, we successfully quantified galactose extracted from the clinical blood spots of newborn babies. Therefore, we suggest that our method is a powerful approach for convenient, economical and time-saving diagnosis of galactosemia in newborn screening.

키워드

저자

• Min-Ah WOO [ Department of Chemical and Biomolecular Engineering, KAIST ]
• Moon Il KIM [ Department of Chemical and Biomolecular Engineering, KAIST. ]
• Hyun Gyu PARK [ Department of Chemical and Biomolecular Engineering, KAIST. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576