Kyung-Ho KANG, Joon-Serk SEO, Jong-Moon CHO, Dong-Il KIM
언어
영어(ENG)
URL
https://www.earticle.net/Article/A144572
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원문정보
초록
영어
F2N78, a human somatic hybrid cell line, has been derived from a fusion of human embryonic kidney 293 (HEK293) cells and Namalwa cells. HEK293 has been widely used as a human host for the effective transient expression of recombinant proteins, and Namalwa is a Burkitt’ lymphoma cell line harboring integrated Epstein-Barr Virus (EBV) genome in its chromosome. F2N78 has been adapted to serum-free suspension condition, so that the transfection could be performed in suspension cultures. In this study, we investigated the transient transfection of F2N78 under suspension condition. Higher transfection efficacy and long-lasting cell viability could be obtained compared with other cell lines such as HEK293 and Chinese hamster ovary (CHO-K1) cells usually used for the expression of foreign genes. Furthermore, the growth characteristics and metabolism of F2N78 cell culture were analyzed using different basal media to find an optimal medium. It was found that a chemically defined media named MPC002 provided the best growth conditions. Optimization of MPC002 for the production of recombinant antibody in F2N78 cells is going to be performed for further study.
키워드
F2N78 (Fusion of HEK293 and Namalwa) cellsHuman somatic hybrid cell lineRecombinant antibody
저자
Kyung-Ho KANG [ Department of Biological Engineering, Inha University, Incheon 402-751, Korea. ]
Joon-Serk SEO [ Department of Biological Engineering, Inha University, Incheon 402-751, Korea. ]
Jong-Moon CHO [ Celltrion, Incheon 406-840, Korea. ]
Dong-Il KIM [ Department of Biological Engineering, Inha University, Incheon 402-751, Korea. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동
간행물
간행물명
한국생물공학회 학술대회
간기
반년간
수록기간
1985~2013
십진분류
KDC 476DDC 576
이 권호 내 다른 논문 / 한국생물공학회 학술대회 2011년도 한국생물공학회 춘계학술발표대회