Earticle

초록

영어

In tissue engineering, immobilization of growth factors or peptides on scaffolds is advantageous for promotion of viability, proliferation, and differentiation of target cells. One such ligand is osteogenic growth peptide (OGP), a short and naturally occurring 14-mer growth factor peptide found naturally in human serum at μmol/L concentration. Here, we designed fusion immobilizing protein, fp-151-OGP, for efficient osteoblast adhesion, based on fusion of mussel adhesive protein (MAP) with OGP. We expressed fp-151-OGP in Escherichia coli system and easily purified by one-step extraction method. Osteoblast behaviors, including attachment and proliferation were investigated on the fp-151- OGP-coated surfaces using mouse pre-osteoblast cell lines (MC3T3-E1). We found that osteoblast on fp-151-OGP-coated surface displayed good attachment and proliferation properties, compared to other commercially available cell adhesion material, poly-L-lysine (PLL). Collectively, our results suggest that the new functional fusion MAP, fp-151-OGP, can be successfully used in bone tissue engineering fields for improving osteoblast growth.

키워드

저자

• Bong-Hyuk CHOI [ Department of Chemical Engineering, Pohang University of Science and Technology, Pohang 790-784, Korea. ]
• Yoo Seong CHOI [ Department of Chemical Engineering, Pohang University of Science and Technology, Pohang 790-784, Korea. ]
• Hyung Joon CHA [ Department of Chemical Engineering, Pohang University of Science and Technology, Pohang 790-784, Korea. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576