This study is focused on analyzing the enhanced hydrogen production by extracted mixed organic compounds from Rhodobacter sphaeroides. In this study, recombinant plasmid was constructed to analyze the effect of hydrogen production by the expression of HupSL hydrogenase isolated from R. sphaeroides in Escherichia coli. Hydrogen production by expression of HupSL hydrogenase from recombinant E. coli was increased 20.9-fold compared to control E. coli. In addition, we found that biohydrogen production from recombinant E. coli was more increased under the mixed organic compounds supplemented condition. The concentration of the ATP as cofactor for hydrogen production from in mixed organic compounds extracted from R. sphaeroides showed about 10-7M, while E. coli extract was about 10-10M. From these results, we conclude that mixed organic compounds from R. sphaeroides have multifunctional potential to increase the fermentation efficiency of microorganism.
키워드
R. sphaeroidesmixed organic compoundsrecombinant E. coliATP
저자
Hyun Jeong LEE [ Graduate School of Semiconductor and Chemical Engineering, Chonbuk National University, 664-14 Deokjin-dong 1Ga Deokjin-Gu Jeonju 561-756, South Korea. ]
Yang-Hoon KIM [ Department of microbiology, Chungbuk National University, 410 Sungbong-Ro, Heungduk-Gu, Cheongju 361-763, South Korea. ]
Jiho MIN [ Graduate School of Semiconductor and Chemical Engineering, Chonbuk National University, 664-14 Deokjin-dong, 1Ga Deokjin-Gu Jeonju 561-756, South Korea. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동
간행물
간행물명
한국생물공학회 학술대회
간기
반년간
수록기간
1985~2013
십진분류
KDC 476DDC 576
이 권호 내 다른 논문 / 한국생물공학회 학술대회 2011년도 한국생물공학회 춘계학술발표대회