Recombinant spider and silkworm have been studied as silk materials for several decades. Even though bees and ants have been focused as new silk-bearing organisms, the research for silk proteins from other organisms is rare. Especially, there is no precedent about silk-like proteins from marine organisms. Comparing with the silk genes from spider and silkworm, we found that marine mussel has silk-like gene that consists of many repeats with abundance of glycine and alanine. We designed mussel-derived silk gene which contains alternating repeated (hydrophobic) and non-repeated regions. Because we observed prominent retardation of cell growth by expression of mussel silk-like protein, fusion strategy with the baculoviral polyhedrin was used to induce insoluble expression of target silk protein. Finally, we could express the mussel silk-like proteins as insoluble form with high cell growth.
키워드
MusselSilk-like proteinBaculoviral polyhedrin
저자
Yun Jung YANG [ Department of Chemical Engineering, Pohang University of Science and Technology, Pohang 790-784, Korea. ]
Yoo Seong CHOI [ Department of Chemical Engineering, Pohang University of Science and Technology, Pohang 790-784, Korea. ]
Hyung Joon CHA [ Department of Chemical Engineering and Ocean Science and Technology Institute, Pohang University of Science and Technology, Pohang 790-784, Korea. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동
간행물
간행물명
한국생물공학회 학술대회
간기
반년간
수록기간
1985~2013
십진분류
KDC 476DDC 576
이 권호 내 다른 논문 / 한국생물공학회 학술대회 2011년도 한국생물공학회 춘계학술발표대회