Citrus peel waste is produced after the extraction of juice, from citrus fruits. The citrus peel waste contains about 27% solid while a 73% of liquid content. The juicy content was used as a substrate for fermentation to produce antimicrobial substance. The organism used for fermentation was Bacillus sp. LS 1-2, isolated from Korean transitional soybean paste foods. It was confirmed as Bacillus sp based on 16S rRNA sequence similarities. LS 1-2 was cultured in citrus-processing-waste containing five Brix as a culture medium and produced an antimicrobial agent against both gram-positives and negatives at adequate culture conditions (pH, Temperature, Culture period and, etc.). An antimicrobial agent produced by LS 1-2 was purified by the consecutive steps of the alcohol precipitation, C-18 cartridges, Recycle preparative HPLC and preparative HPLC. The active compound was not sensitive to lipase, proteinase K, amylase, catalase but was affected by pronase E. In order to evaluate its commercial value, the minimum inhibitory concentration of purified antim icrobial agent from Bacillus sp. LS 1-2 was compared with commercialized antimicrobial agents (Ampicillin) against Staphylococcus aureus and Escherichia coli O-157.
키워드
BacillusCitrusAntimicrobial activityprobiotics
저자
Jihyuk YU [ Dept. of Biological Engineering, Inha University, Incheon, Korea. ]
Dung Hoang NGUYEN [ Dept. of Biological Engineering, Inha University, Incheon, Korea. ]
Hyang-Bok LEE [ Dept. of Biological Engineering, Inha University, Incheon, Korea. ]
Wooyoung CHOI [ Dept. of Biological Engineering, Inha University, Incheon, Korea. ]
Eun-Ki KIM [ Dept. of Biological Engineering, Inha University, Incheon, Korea. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동
간행물
간행물명
한국생물공학회 학술대회
간기
반년간
수록기간
1985~2013
십진분류
KDC 476DDC 576
이 권호 내 다른 논문 / 한국생물공학회 학술대회 2011년도 한국생물공학회 춘계학술발표대회