Thai-Hoang LE, Sung-Kyu LEE, Yang-Hoon KIM, Jiho MIN
언어
영어(ENG)
URL
https://www.earticle.net/Article/A144549
※ 원문제공기관과의 협약기간이 종료되어 열람이 제한될 수 있습니다.
원문정보
초록
영어
Toxicity of two common pesticides (e.g., glyphosate and methidathion) and their impacts on the gene expression in non-target organisms were studied in this work using a water flea, Daphnia magna. Particularly, we investigated both the acute (24h) and chronic (21d) effects of these chemicals on physiological responses such as viability, growth, and reproduction as well as molecular responses such as gene expression in D. magna. Through RT-PCR, the expression of five genes selected as representative biomarkers including Arnt, Vtg, CYP4, CYP314 and Dhb was analyzed in D. magna exposed to glyphosate and methidathion. Our results showed that the expression of these genes in D. magna were significantly down-regulated in response to the LC5, LC10, LC20, and LC50 concentrations of pesticides for short-time (24h) exposure. Among five examined genes, Cyp4 and Cyp314 genes, expressed differently in exposure of glyphosate and methidathion, respectively. This revealed that these two genes could be considered as potential biomarkers for the acute toxicity of glyphosate and methidathion. Interestingly, expression of these five genes in D. magna tended to increase when culturing neonates in the medium fed with some low concentrations (e.g., 1/10, 1/50, and 1/100 LC50) of both pesticides for a long-time (21d) experiment. Therefore, this finding suggested that the acute toxicity of glyphosate and methidathion may cause some damages on the DNA function in D. magna which lead to the decrease of expression level of the five selected genes while the chronic toxicity of these chemicals may stimulate some acclimation mechanism to help organisms to overcome the adverse impacts from the toxic environments.
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동
간행물
간행물명
한국생물공학회 학술대회
간기
반년간
수록기간
1985~2013
십진분류
KDC 476DDC 576
이 권호 내 다른 논문 / 한국생물공학회 학술대회 2011년도 한국생물공학회 춘계학술발표대회