Earticle

초록

영어

Fermentative production of liquid biofuel as alternatives to gasoline such as ethanol and butanol has gained interest due to both economic and environmental reasons. Butanol that has better fuel properties than ethanol is now considered as one of the most promising next generation biofuel. At the same time, marine biomass such as macroalgae, can provide a high-yield source of biofuels without considering the food crisis or consumption of water and fertilizer. (1,2) Although the carbohydrate composition of macroalgae is more complicated, there is no lignin found in most of macroalgae, which can facilitate production of butanol trough a consolidated bioprocessing (CBP) which features hydrolase production, polymeric carbonhydrate hydrolysis and fermentation in one step. (3) As solventogenic clostridia are capable to produce a variety of extracellular enzymes to degrade polysaccharides, macroalgae fermentation seems to be more suitable for butanol production rather than ethanol production by yeast strains. In this study, red brown and grown macroalgae, Laminaria japonica, Pachymeniopsis elliptica, Chondrus ocellatus, Enteromorpha compressa and Gelidium amansii were used for butanol fermentation with different hydrolysis treatment. Acid or enzyme treatment was applied to the fermentation of L. japonica, P. elliptica, C. ocellatus and E. compressa, while acid pretreatment modified from NREL hydrolysis method for lignocellulosic materials (4) was applied prior to the fermentation of Gelidium amansii.Clostridium beijerinckii NCIMB 8052, a well-known solventogenic clostridium, was used in this research. L. japonica and P. elliptica produced the most reducing sugar of 6.27 ± 0.46 g/L and 6.22 ± 0.12 g/L, respectively. 4 ml and 20 ml fermentation tests were performed in the hydrolysate of each kind of macroalgae. In the case of L. japonica, it was noticeable that water soluble material without any pretreatment was found to be a fine culture medium for butanol production, giving a butanol product of 0.27 g/L in 20 ml culture. The supernatant produced the highest butanol production of 0.34 g/L in 20 ml culture was obtained from P. elliptica hydrolysates with combination treatment of acid and enzyme. Fermentation of the Gelidium amansii hydrolysate was performed in a 200 ml culture after 5 ml and 50 ml cultures were tested. In several runs of fermentation which were conducted under different concentrations of the hydrolysate, a maximum amount of 2.71 g/L of solvents (2.18 g/L of butanol) production, and the high level of acids (acetate and butyrate) production, 8.67 g/L, were obtained at 36 h after inoculation. It was also found that Clostridium beijerinckii can grow and metabolize brown algae Laminaria japonica, whose carbonhydrate composition are alginate, cellulose, laminarin and mannitol without any pretreatment or supplyment. 50 ml and 500 ml of fermentation were performed in a concentration of 100 g/L (dry wt/v) brown algae media resulting in 1.2 g/L of butanol at the end of the fermentation (36h).On the other hand, many different carbohydrates available in macroalgae were test for butanol production to examine carbohydrate preference or utilization by C. beijerinckii in order to provide more information on utilization of macroalgae as substrate. The aim of this study is to examine potential of butanol production using macroalgae and to provide a basis for biofuel production from marine biomass.

키워드

저자

• Pan LI [ Dept. of Chemical Engineering, Sungkyunkwan University, Suwon, 440-746. ]
• Byungwoo KIM [ Dept. of Chemical Engineering, Sungkyunkwan University, Suwon, 440-746. ]
• Jieun LEE [ Dept. of Chemical Engineering, Sungkyunkwan University, Suwon, 440-746. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576