Earticle

초록

영어

Colorimetric detection of nucleic acids has attracted considerable research attention due to many advantages as compared to the conventional gel based assays, such as rapid visual detection, low cost and easy implementation to point-of-care testing (POCT) facilities. Along this line, we developed a new and simple label-free colorimetric detection method of target DNA utilizing the peroxidase mimicking activity of magnetic nanoparticles (MNPs), which relies on the target DNA-induced shielding action against the peroxidase activity. PCRamplified sample solution is mixed with unmodified MNPs followed by addition of colorimetric reaction solution containing colorimetric substrates and hydrogen peroxidase (H2O2). In the presence of target DNA, the am plified DNA products diminished the accessibility of positively charged substrate, OPD toward MNPs in a supernatant and the direct contact of the substrate with MNPs by adsorbing onto the surface of MNPs through electrostatic interactions. This simple but very efficient colorimetric strategy was successfully demonstrated by correctly diagnosing Chlamydia infection using a real human urine sample. Since the results can be clearly seen by the naked eye without any sophisticated instrumentation, this method has great potential as a POCT sensor for the verification of the amplified nucleic acids.

키워드

저자

• Ki Soo PARK [ Chemical and Biomolecular Engineering, KAIST, Daejeon 305-701. ]
• Moon Il KIM [ Chemical and Biomolecular Engineering, KAIST, Daejeon 305-701. ]
• Hyun Gyu PARK [ Chemical and Biomolecular Engineering, KAIST, Daejeon 305-701. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576