Earticle

초록

영어

In this study, a steady supply of ATP for cell-free protein synthesis was achieved by using starch as an energy source. Glycogen phosphorylase (GlgP) activity in the E. coli cell extract caused continuous release of glucose, which was then metabolized to regenerated ATP molecules. Compared to the previously reported methods employing glucose as the primary energy source, the use of starch alleviated the problem of a pH drop during the cell-free protein synthesis reaction, thereby allowing a prolonged reaction period. As a result, after 12 hours of incubation, approximately 1.7±0.1 mg/mL of the target protein accumulated in the reaction mixture. In addition, most likely due to the relatively homeostatic maintenance of the reaction conditions and controlled rate of translation, substantially larger amounts of proteins were obtained in a soluble and functional form.

키워드

저자

• Ho-Cheol KIM [ Department of Fine Chemical Engineering and Applied Chemistry, Chungnam National University, Daejeon 305-764, Korea. ]
• Tae-Wan KIM [ Department of Chemical Engineering, University of Califonia, Berkeley, CA 94720, USA. ]
• Kyung-Ho LEE [ Department of Fine Chemical Engineering and Applied Chemistry, Chungnam National University, Daejeon 305-764, Korea. ]
• Yong-Chan KWON [ Department of Fine Chemical Engineering and Applied Chemistry, Chungnam National University, Daejeon 305-764, Korea. ]
• Ka-Young LEE, [ Department of Fine Chemical Engineering and Applied Chemistry, Chungnam National University, Daejeon 305-764, Korea. ]
• Ji-Yeong PARK [ Department of Fine Chemical Engineering and Applied Chemistry, Chungnam National University, Daejeon 305-764, Korea. ]
• Dong-Myung KIM [ Department of Fine Chemical Engineering and Applied Chemistry, Chungnam National University, Daejeon 305-764, Korea. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576