All eukaryotic cells contain functional cell organelles such as lysosomes (LY) and peroxisomes (PE). In our study, it has tried to characterize the suitable conditions to separate subcellular distribution of lysosomal and peroxisomal proteins in eukaryotic cells and evaluate different in vitro function of each cell organelle. To certify to organelles' special function, several chemicals were used to stimulate in vitro function to LY or PE isolated from Saccharomyces cerevisiae. We found that in comparison with control, the number of and fluorescent intensity of LY were increased 3.4, 5.5 or 2.0 folds in HeLa cells exposed by 1unit/ml glucose oxidase, salicylic acid or 6-hydroxydopaime (6-OHDA), respectively. It maybe because these chemicals contain the hydroxy functional group in the structures. Additionally, in vitro antimicrobial and anticancerous activity of LY was enhanced over 2 folds when exposed by 6-OHDA. However, PE were 0.8 or 2.0 folds inactivated by exposure of phenylhydrazine (PHD) or sodium azide, therefore melanin degradation effect of peroxisomal extract was reduced less than 2 folds by PHD, based on the reduction of melanin pigmentary intensity. Therefore, our results provide insight for the application of LY and PE extract as useful asgents for antimicrobial, anticacerous agents and treatment of hyperpigmentation disorders.
키워드
lysosomesperoxisomesIn vitro function
저자
Jihee YOON [ Graduate School of Semiconductor and Chemical Engineering, Chonbuk National University, 664-14 Deokjin-dong, 1Ga Deokjin-Gu Jeonju 561-756. ]
Yang-Hoon KIM [ Department of Microbiology, Chungbuk National University, 410 Sungbong-Ro, Heungduk-Gu, Cheongju 361-763. ]
Jiho MIN [ Graduate School of Semiconductor and Chemical Engineering, Chonbuk National University, 664-14 Deokjin-dong, 1Ga Deokjin-Gu Jeonju 561-756. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동
간행물
간행물명
한국생물공학회 학술대회
간기
반년간
수록기간
1985~2013
십진분류
KDC 476DDC 576
이 권호 내 다른 논문 / 한국생물공학회 학술대회 2011년도 한국생물공학회 춘계학술발표대회