Changsoo Lee, Minjae Kim, Seung Gu Shin, Seokhwan Hwang
언어
영어(ENG)
URL
https://www.earticle.net/Article/A144507
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원문정보
초록
영어
A major value of anaerobic digestion is the production of fuel-grade biogas formed as the end product during degradation of organic material. Methanogens participate in the terminal step of anaerobic digestion, methanogenesis, and play a key role in generation of methane. Since methanogenesis is the rate-limiting step in most anaerobic processes, lots of works on the process optimization study focused on the methanogenesis step have been made. Those have been, however, confined to modification of process and optimization of operating conditions. In order to overcome the innate biological limit, therefore, molecular biological approaches should be concurrent with the process optimization study. M. thermophila is one of the most metabolically diverse methanogens in that it utilizes the methyl groups of acetate, methanol, or methylamines for growth and methanogenesis [1]. Genetic manipulation of an aceticlastic methanoarchaeon Methanosarcina thermophila was performed. A metabolic gene which encodes carbonic anhydrase(ca) from M. thermophila was overexpressed in M. thermophila TM-1 host strain. The recombinant plasmid used to transfer ca into the host cell, pMC1, was maintained in approximately four copies per cell. This result corresponded to the previously reported results for methanogenic natural plasmids. The recombinant strain showed about 2.4-fold higher expression level of ca than the wild-type strain. Corresponding to this result, the carbonic anhydrase activity was also about 2.1-fold higher in the recombinant strain. These results indicated that the pMC1 was well-functioning in the recombinant M. thermophila host cells and, as a result, the elevation of carbonic anhydrase activity was induced by over expression of ca.
키워드
MethanogenRecombinationCarbonic Anhydrase
저자
Changsoo Lee [ School of Environmental Science and Engineering, Pohang University of Science and Technology (POSTECH) ]
Minjae Kim [ School of Environmental Science and Engineering, Pohang University of Science and Technology (POSTECH) ]
Seung Gu Shin [ School of Environmental Science and Engineering, Pohang University of Science and Technology (POSTECH) ]
Seokhwan Hwang [ School of Environmental Science and Engineering, Pohang University of Science and Technology (POSTECH) ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동
간행물
간행물명
한국생물공학회 학술대회
간기
반년간
수록기간
1985~2013
십진분류
KDC 476DDC 576
이 권호 내 다른 논문 / 한국생물공학회 학술대회 2011년도 한국생물공학회 춘계학술발표대회