The nanoparticles (NPs) have been used in a variety of fields due to their authentic physicochemical properties. Biological or environmental toxicity of NPs, however, has not been fully understood. In this study, the toxicity of water stable fullerene colloids, nC60 and fullerol, C60-OH NPs was assessed in Caenorhabditis elegans through the measurement of lifespan and brood size. The water stable colloids form of nC60 NPs are prepared through the long-term exposure of fullerene in THF followed by repeated dialysis with water. C. elegans is a free-living soil nematode feeding on bacteria such as Escherichia coli. We have found that the oral-administrated NPs were accumulated in the animals for several days after feeding the mixture of bacterial food and nC60 or C60-OH NPs to the L4 larvas of C. elegans. The fullerol NPs reduced the viability of animals while no decrease in survival was found in the animals fed with nC60. It was also found that both nC60 and C60-OH diminished the reproduction of C. elegans. In order to illuminate the genetic mechanism of toxicity induced by those NPs, the assessment of viability and fertility was carried out using the strains mutated in oxidative stress or programmed cell death, apoptosis regulators such as CED-3, CED-4, and EGL-1.
키워드
nanoparticlesfullerenefullerolC. elegans
저자
Yun Jeong CHA [ Dept. of Food and Nutrition, Myongji University, Yongin, Kyonggi 449-728. ]
Shin Sik CHOI [ Dept. of Food and Nutrition, Myongji University, Yongin, Kyonggi 449-728. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동
간행물
간행물명
한국생물공학회 학술대회
간기
반년간
수록기간
1985~2013
십진분류
KDC 476DDC 576
이 권호 내 다른 논문 / 한국생물공학회 학술대회 2011년도 한국생물공학회 춘계학술발표대회