Human basophilic KU812F cells express a high affinity for IgE receptor, FcεRI, which plays an important role in immunoglobulin E (IgE)-mediated allergic reactions. Using the human basophilic KU812F cells, we assessed the inhibitory effects of the ethanolic extract from Chrysanthemum zawadsaki(CZ) on FcεRI α chain expression. Flow cytometric analysis showed that cell surface FcεRI α chain expression was suppressed when the cells were cultured with CZ extract. RT-PCR analysis showed that the mRNAs for FcεRI α chain decreased by the treatment of CZ extract. Moreover, CZ extract was also observed to result in a reduction in the release of histamine from anti-FcεRI antibody (CRA-1)-stimulated cells. These results suggest that CZ extract may exert anti-allergic activity through down-regulation of FcεRI α chain expression and a subsequent decrease in histamine release.
목차
Abstract 서론 재료 및 방법 시료 및 재료 시료의 추출 세포배양, 시료처리 세포독성 측정 세포표면 FcεRI α chain 발현량 측정 FcεRI α chain mRNA 발현량 측정 히스타민 측정 통계학적 분석 결과 및 고찰 KU812F 세포에 대한 구절초 추출물의 세포독성효과 세포표면 FcεRI α chain 발현 억제 효과 세포표면 FcεRI α chain mRNA 발현 억제 효과 히스타민 유리 억제효과 요약 문헌
키워드
Chrysanthemum zawadskii var. latilobumFcεRIhistamine