늙은 호박 부위별 추출물의 항산화 효과 및 생리활성에 관한 연구
Antioxidant Effects and Physiological Activities of Pumpkin (Cucurbita moschata Duch.) Extract from Different Aerial Parts
This study investigated the antioxidant effects and physiological activities of extract from various parts of pumpkin (Cucurbita moschata Duch.) such as leaf, flesh, skin and seed. Different dried parts of C. moschata were boiled in ethanol for three hours, evaporated, and lyophilized with a freeze-dryer. Total polyphenol contents were the highest in leaf (29.62±0.88 mg GAE/g DM). The same result was observed in the measurement of the flavonoid content. The leaf extract of C. moschata had stronger DPPH radical-scavenging activities and ABTS-radical scavenging activities than any other part. Leaf extract of C. moschata was found to have the highest ferric-reducing antioxidant power (FRAP) value. Meanwhile, at 0.1 mg/mL of all extracts higher degree of tyrosinase inhibitory effects than kojic acid was observed (leaf; 49.58%, flesh; 40.20%, skin; 41.30%, seed; 36.79% and kojic acid; 20.70%). The glycation inhibitory activity decreased in the order leaf>skin>flesh>seed. The α-glucosidase inhibitory activity of seed extract was the highest with a value of 63.58% at the concentration of 1 mg/mL.
목차
Abstract 서론 재료 및 방법 늙은 호박 부위별 추출 및 조제 일반성분 및 수율 총 폴리페놀 화합물 함량 총 플라보노이드 화합물 함량 DPPH radical 소거 활성 ABTS radical 소거 활성 Ferric-reducing antioxidant power(FRAP)에 의한 항산화능측정 Tyrosinase 활성 저해 효과 최종당화산물 생성 억제 효과 α-glucosidase 활성 억제 효과 통계 처리 결과 및 고찰 일반성분 및 수율 총 폴리페놀 화합물 함량 총 플라보노이드 화합물 함량 DPPH radical 소거 활성 ABTS radical 소거 활성 Tyrosinase 효소 활성 억제 효과 최종당화산물의 제조 및 확인 α-Glucosidase 억제 효과 요약 문헌