Earticle

초록

영어

The pig has been considered to serve as an appropriate model of human disease. Therefore, establishment of porcine embryonic stem cell lines is important. The purpose of the present study was to further work in this direction. We produced porcine parthenogenetic embryos, and separately aggregated two of each of two-cell (2×2), four-cell (2×4), and eight-cell (2×8) embryos derived by parthenogenesis. After culture for 4 days, the developmental ability of the aggregates and total blastocyst cell numbers were evaluated. The percentage of blastocysts was significantly higher in both 2×4- and 2×8-aggregated embryos (58.3±1.9% and 37.2±2.8%, respectively) than in the control or 2×2-aggregated embryos (23.6±1.1% and 12.5±2.4%, respectively). Total blastocyst cell numbers were increased in the 2×4- and 2×8-aggregated embryos (by 44±3.0% and 45±3.3%, respectively) compared with those of control or 2×2-aggregated embryos (30.5±2.1% and 30.7±2.6%, respectively; p<0.05). The levels of mRNA encoding Oct-4 were higher in both the 2×4- and 2×8-aggregated embryos than in the control. When blastocysts derived from 2×4- aggregated embryos or intact normal embryos were cultured on mouse embryonic fibroblast feeder cells to obtain porcine stem cells, blastocysts from aggregated embryos formed colonies that were better in shape compared with those derived from intact blastocysts. Together, the data show that aggregation of porcine embryos not only improves blastocyst quality but also serves as an efficient procedure by which porcine embryonic stem cells can become established.

목차

ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
  Chemicals
  In Vitro Maturation
  Activation of Porcine Oocytes and In Vitro Culture
  Aggregation of Embryos and In Vitro Culture
  Preparation of Feeder Cells and Establishment of EmbryonicStem Cells
  Real-Time Quantitative Polymerase Chain Reaction
  Statistical Analysis
 RESULTS
  Developmental Ability of Aggregated ParthenogeneticEmbryos
  Total Cell Numbers in Aggregated and Intact Embryos
  Analysis of Oct-4 Transcription in Aggregated Blastocysts
  Establishment of Porcine Embryonic Stem Cells
 DISCUSSION
 REFERENCES

키워드

저자

• Jin Yu Zhang [ Department of Animal Science & Biotechnology, Research Center for Transgenic Cloned Pigs, Chungnam National University, Daejeon 305-764, Korea ]
• Yun Fei Diao [ Department of Animal Science & Biotechnology, Research Center for Transgenic Cloned Pigs, Chungnam National University, Daejeon 305-764, Korea ]
• Reza K. Oqani [ Department of Animal Science & Biotechnology, Research Center for Transgenic Cloned Pigs, Chungnam National University, Daejeon 305-764, Korea ]
• Je Sok Wo [ National Institute of Animal Science, Suwon 441-706, Korea ]
• Dong Il Jin [ Department of Animal Science & Biotechnology, Research Center for Transgenic Cloned Pigs, Chungnam National University, Daejeon 305-764, Korea ] Corresponding author

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간행물

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• 간행물명

  Reproductive & developmental biology

• 간기

  계간

• pISSN

  1738-2432

• eISSN

  2288-0151

• 수록기간

  1977~2018

• 십진분류

  KDC 527 DDC 636