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Nuclear Modeling and Developmental Potential of Bovine Somatic Nuclear Transfer Embryos Cloned by Two Different Activation Methods

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  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Reproductive & developmental biology 바로가기
  • 통권
    Volume 35 No 1 (2011.03)바로가기
  • 페이지
    pp.105-113
  • 저자
    Byeong-Gyun Jeon, Gyu-Jin Rho
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A140489

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원문정보

초록

영어
The present study investigated the nuclear remodeling, development potential with telomerase activity and transcription level of X-linked genes (ANT3, HPRT, MeCP2, RPS4X, XIAP, XIST and ZFX) in the bovine somatic cell nuclear transfer (SCNT) embryos using two different fusion and activation methods. Female adult fibroblasts were injected into perivitelline space of in vitro matured oocytes. The oocyte-nucleus complexes were fused and followed by immediately either activated (Group 1), or activated at 1 h post-fusion (hpf) (Group 2), respectively. The incidence of normal premature chromosome condensation (PCC) at 1 hpf was slightly increased in the Group 2, compared to those of Group 1, but there was no significant (p<0.05) difference. The incidence of normal pronucleus (PN) and chromosome spread at 5 and 18 hpf were significantly (p<0.05) higher in the Group 2 than those of Group 1. The cleavage rate to 2-cell stage, developmental rate to blastocyst stage, and the mean number of total and ICM cell numbers were significantly (p<0.05) higher in the Group 2, compared to those of Group 1. Level of telomerase activity was significantly (p<0.05) higher in the SCNT blastocysts of Group 2, compared to those of Group 1. Transcript levels of HPRT, MeCP2 and XIST were not significantly (p<0.05) different between blastocysts of Group 1 and 2. However, transcript level of ANT3, RPS4X, XIAP and ZFX were significantly (p<0.05) up-regulated in the SCNT blastocysts of Group 2, compared to those of Group 1. Taken together, it is concluded that oocyte activation at 1 hpf induces the enhanced developmental potential by efficient nuclear remodeling and subsequent facilitation of the nuclear reprogramming of bovine SCNT embryos.

목차

ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
  Media and Chemicals
  Preparation of Donor Cells
  Preparation of Recipient Cytoplasms
  Nuclear Transfer and Oocyte Activation
  In Vitro Culture of Embryos
  Immunofluorescent Staining of Embryos
  Absolutely Quantitative Analysis of Transcripts by RealTime RT-PCR
  Relative-quantitative Telomerase Repeat AmplificationProtocol (RQ-TRAP)
  Statistical Analysis
 RESULTS
  Developmental Potential of SCNT Embryos
  Relative Telomerase Activity (RTA) of SCNT Embryos
  Transcript Levels of X-linked Genes in SCNT Embryos
 DISCUSSION
 ACKNOWLEDGEMENTS
 REFERENCES

키워드

Somatic cell nuclear transfer Oocyte activation Nuclear remodeling Telomerase activity X-linked gene expression

저자

  • Byeong-Gyun Jeon [ OBS/Theriogenology and Biotechnology, College of Veterinary Medicine, Institute of Animal Medicine ]
  • Gyu-Jin Rho [ OBS/Theriogenology and Biotechnology, College of Veterinary Medicine, Research Institute of Life Science, Gyeongsang National University, Jinju 660-701, Korea ] Corresponding author

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Reproductive & developmental biology
  • 간기
    계간
  • pISSN
    1738-2432
  • eISSN
    2288-0151
  • 수록기간
    1977~2018
  • 십진분류
    KDC 527 DDC 636

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