Earticle

초록

영어

Transducin-like enhancer protein 1(TLE-1) is protein associated with cell proliferation. This study analyzed change of TLE-1 mRNA expression during in vivo and in vitro maturation in porcine oocytes. Oocytes and granulose cells were collected from follicles of <2 mm, 2～6 mm and >6 mm in diameter in slaughtered pig’s ovaries. Oocytes collected from follicles of 2～6 mm in diameter were used after in vitro maturation for 0, 10, 20 and 44 h. Cumulus cells and granulose cells were collected after treatment with hyaluronidase. In results, TLE-1 mRNA expression in oocytes collected from follicle >6 mm in diameter is increased, TLE-1 RNA expression in cumulus cells and granulosa cells from follicles <2 mm, 2 mm 6 mm and >6 mm in diameter. However, there is no significant difference. On the other hand, TLE-1 mRNA expression from oocytes cultured for 10 h and 44 h is increased, TLE-1 mRNA in cumulus cells cultured for 10 h is significant increased(p<0.05) than other culture periods. In conclusion, these results show that TLE-1 is expressed in all cell types of oocytes, cumulus cells and granulose cells, and associated with oocyte maturation.

목차

ABSTRACT
 서론
 재료 및 방법
  난포난자의 회수
  과립막세포의 회수
  통계 처리
 결과
 고찰
 감사의 글
 인용문헌

키워드

저자

• 장예진 [ Ye-Jin Jang | 강원대학교 동물생명과학대학 ]
• 김동우 [ Dong-Woo Kim | 강원대학교 동물생명과학대학 ]
• 이용승 [ Yong-Seung Lee | 강원대학교 동물생명과학대학 ]
• 정희태 [ Hee-Tae Cheong | 강원대학교 수의과대학 ]
• 양부근 [ Boo-Keun Yang | 강원대학교 동물생명과학대학 ]
• 박춘근 [ Choon-Keun Park | 강원대학교 동물생명과학대학 ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Reproductive & developmental biology

• 간기

  계간

• pISSN

  1738-2432

• eISSN

  2288-0151

• 수록기간

  1977~2018

• 십진분류

  KDC 527 DDC 636