연구논문
제 2형 당뇨쥐에서 동충하초로부터 생산된 세포외 다당류의 항당뇨 효과
Anti-diabetic effect of the exopolysaccharides (EPS) produced from Cordyceps sinensis on ob/ob mice
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- 발행기관
- 한국생물공학회 바로가기
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- 간행물
- KSBB Journal KCI 등재 바로가기
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- 통권
- 제26권 제1호 (2011.02)바로가기
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- 페이지
- pp.33-40
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- 저자
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- 언어
- 한국어(KOR)
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- URL
- https://www.earticle.net/Article/A139406
※ 원문제공기관과의 협약기간이 종료되어 열람이 제한될 수 있습니다.
원문정보
초록
- 영어
- Anti-diabetic effect of the exopolysaccharides (EPS) produced from submerged mycelial culture of Cordyceps sinensis (Cs) was studiedin a type II diabetic animal model (C57BL/6J ob/ob). This study was designed to determine whether Cs-EPS improves clinical symptoms of type II diabetes in ob/ob mice. After Cs-EPS treatment at doses of 200 mg/kg body weight, the fasting blood glucose levels decreased by 47% after 7 weeks compared with those of the control mice. According to the oral glucose tolerance test, the glucose levels recovered its baseline after 120 min in Cs-EPS-treated mice, although the blood glucose levels increased significantly after 30 min. On the other hand, the control group (not-treated) did not recovered its initial level of glucose after 120 min. Furthermore, food intake, body weight, total plasma cholesterol and triglyceride concentrations in ob/ob mice treated with Cs-EPS were significantly decreased, compared with those in control ob/ob mice. Cs-EPS treatment increased significantly the plasma insulin level and the expression of leptin mRNA in adipose tissue of Cs-EPS-treated ob/ob mice. From these results, it is demonstrated that Cs-EPS could be effective for regulating normal blood glucose levels by increasing the amounts of plasma insulin and leptin expression in ob/ob mice, indicating that this compound could be a candidate material as a dietary supplement to control hyperglycemia in patients suffering from type II diabetes.
목차
Abstract
1. Introduction
2. Materials andMethods
2.1. Microorganism
2.2. Inoculum preparation and culture condition
2.3. Preparation of EPS
2.4. Purification and content determination of EPS
2.5. Animals and breeding conditions
2.6. Experimental design
2.7. Oral glucose tolerance test
2.8. Analytical measurements
2.9. RNA isolation
2.10. RT-PCR analysis
2.11. Western blot analysis
2.12. Statistical analysis
3. Results and Discussion
3.1. Composition analysis of Cs-EPS
3.2. Effect of EPS on fasting blood glucose levels
3.3. Effect of EPS on oral glucose tolerance test
3.4. Effect of Cs-EPS on plasma insulin levels
3.5. Effect of EPS on plasma lipids and cholesterol
3.6. Effect of Cs-EPS on leptin expression
Acknowledgement
References
키워드
Anti-diabetic activity Cordyceps sinensis exopolysaccharide leptin ob/ob mice저자
간행물 정보
발행기관
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- 발행기관명
- 한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
- 설립연도
- 1984
- 분야
- 공학>생물공학
- 소개
- 이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다 1. 생물공학 분야의 발전을 위한 연구 협력 2. 생물공학의 실용화를 촉진시키기 위한 산학 협동 3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최 4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간 5. 생물공학 발전을 위한 정책 건의 6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동
간행물
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- 간행물명
- KSBB Journal
- 간기
- 격월간
- pISSN
- 1225-7117
- 수록기간
- 2009~2013
- 십진분류
- KDC 476 DDC 576
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